Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  :10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
In the United States (and many other places) only a physician can prescribe these drugs, and even so, it will only be prescribed to patients suffering from a medical problem which needs steroids for treatment. In case you were offered steroids by anybody besides a physician, make sure you get in touch with the police. In the same way, if you were charged with either selling or making use of steroids, it is best to hire a lawyer immediately. Just like other controlled substances such as “crystal meth” and “crack,” steroids are a controlled substance. Although a person might not experience the high that can be caused from the other drugs, the conviction for the illegal use, possession, or supply of these drugs is usually equally as harsh.
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